Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells

J Microbiol Methods. 2004 Nov;59(2):289-92. doi: 10.1016/j.mimet.2004.07.001.

Abstract

The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Benzenesulfonates / chemistry*
  • Benzenesulfonates / metabolism
  • Caco-2 Cells
  • Candida albicans / physiology*
  • Cell Adhesion / physiology*
  • Enterocytes / microbiology*
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • Humans
  • Microscopy, Fluorescence / methods*
  • Quinolinium Compounds / chemistry*
  • Quinolinium Compounds / metabolism

Substances

  • Benzenesulfonates
  • Fluorescent Dyes
  • Quinolinium Compounds
  • C.I. Fluorescent Brightening Agent 28