Low-molecular-weight peptides derived from extracellular matrix as chemoattractants for primary endothelial cells

Endothelium. May-Aug 2004;11(3-4):199-206. doi: 10.1080/10623320490512390.

Abstract

The development of synthetic and naturally occurring scaffolds for tissue engineering applications has included strategies to promote attachment of specific cell types, control the rate of scaffold degradation, encourage angiogenesis, or otherwise modulate the host response. We have reported that bioscaffolds developed from porcine small intestinal submucosa (SIS) facilitate the constructive remodeling of tissues and recruit marrow-derived cells that persist long after the acute inflammatory stages have resolved. We have not yet determined which cells are recruited, the eventual fate of these cells, or via what mechanisms the events occur. We now have analyzed various molecular weight fractions of acid-hydrolyzed SIS by both functional and morphologic methods and have determined that fraction 4 (5 to 16 kDa) possesses chemoattractant activity for primary murine adult liver, heart, and kidney endothelial cells in vitro. Addition of fraction 4 to Matrigel plugs promoted in vivo vascularization when the plugs were implanted subcutaneously in mice. These results indicate that small-molecular-weight peptides derived from the degradation of porcine SIS are biologically active in the recruitment of murine endothelial cells in vitro and in vivo.

MeSH terms

  • Angiogenesis Inducing Agents / isolation & purification
  • Angiogenesis Inducing Agents / pharmacology*
  • Animals
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology
  • Cells, Cultured
  • Chemotactic Factors / isolation & purification
  • Chemotactic Factors / pharmacology*
  • Chemotaxis / drug effects*
  • Chemotaxis / physiology
  • Collagen / pharmacology
  • Drug Combinations
  • Endothelial Cells / drug effects*
  • Endothelial Cells / metabolism
  • Extracellular Matrix Proteins / chemistry
  • Extracellular Matrix Proteins / metabolism
  • Extracellular Matrix Proteins / pharmacology*
  • Intestinal Mucosa / chemistry
  • Intestinal Mucosa / metabolism
  • Laminin / pharmacology
  • Mice
  • Mice, Transgenic
  • Molecular Weight
  • Neovascularization, Physiologic / drug effects
  • Neovascularization, Physiologic / physiology
  • Peptides / isolation & purification
  • Peptides / pharmacology*
  • Proteoglycans / pharmacology
  • Sus scrofa
  • Tissue Engineering / methods

Substances

  • Angiogenesis Inducing Agents
  • Chemotactic Factors
  • Drug Combinations
  • Extracellular Matrix Proteins
  • Laminin
  • Peptides
  • Proteoglycans
  • matrigel
  • Collagen