Differential expression and function of IgA receptors (CD89 and CD71) during maturation of dendritic cells

J Leukoc Biol. 2004 Dec;76(6):1134-41. doi: 10.1189/jlb.0204101. Epub 2004 Sep 15.

Abstract

Dendritic cells (DC) are the most efficient antigen-presenting cells residing in mainly peripheral tissues. Antigen uptake by DC is particularly efficient, being mediated by various receptors such as lectin, scavenger receptors, and Fc receptors (FcRs). Immunoglobulin A (IgA) is part of the first-line immune barrier in mucosae, where DC are numerous. A member of the FcR family, FcalphaRI, is expressed on interstitial DC. We report here that monocyte-derived DC (Mo-DC) express another IgA receptor (IgA-R), the transferrin receptor (TfR), even in the absence of DC proliferation in vitro. Upon incubation with inflammatory cytokines such as tumor necrosis factor alpha and interleukin (IL)-1beta or maturating agents (lipopolysaccharide, CD40 ligand), FcalphaRI and TfR expression on Mo-DC was specifically up-regulated, whereas FcgammaRs and FcepsilonRI expression was down-regulated. Both IgA-Rs were functional, being able to mediate endocytosis by immature and activated Mo-DC. Although FcalphaRI internalized IgA complexes on both types of DC, TfR was only able to mediate IgA complex internalization by immature cells. Cross-linking of FcalphaRI but not of TfR resulted in up-regulation of major histocompatibility complex (MHC) class II/CD86 expression and secretion of IL-10 and IL-12 by immature Mo-DC. Moreover, in activated Mo-DC, cross-linking of FcalphaRI could up-regulated MHC class II/CD86 and triggered IL-10 secretion. Our findings led us to propose that FcalphaRI expressed by interstitial-type DC could play a critical role to sample IgA-recognized antigens and also during DC activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen Presentation / immunology
  • Antigens, CD / drug effects
  • Antigens, CD / immunology*
  • Antigens, Differentiation, B-Lymphocyte / drug effects
  • Antigens, Differentiation, B-Lymphocyte / immunology*
  • B7-2 Antigen
  • Cell Differentiation / drug effects
  • Cell Differentiation / immunology*
  • Cells, Cultured
  • Cytokines / immunology
  • Cytokines / pharmacology
  • Dendritic Cells / drug effects
  • Dendritic Cells / immunology*
  • Endocytosis / drug effects
  • Endocytosis / immunology
  • Humans
  • Immunoglobulin A / immunology
  • Interleukin-10 / immunology
  • Interleukin-10 / metabolism
  • Interleukin-12 / immunology
  • Interleukin-12 / metabolism
  • Lipopolysaccharides / pharmacology
  • Membrane Glycoproteins / immunology
  • Monocytes / immunology*
  • Receptors, Fc / drug effects
  • Receptors, Fc / immunology*
  • Receptors, IgE / drug effects
  • Receptors, IgE / immunology
  • Receptors, IgG / drug effects
  • Receptors, IgG / immunology
  • Receptors, Transferrin / immunology
  • Up-Regulation / drug effects
  • Up-Regulation / immunology

Substances

  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • B7-2 Antigen
  • CD71 antigen
  • CD86 protein, human
  • Cytokines
  • FCGR1A protein, human
  • Fc(alpha) receptor
  • IgA receptor
  • Immunoglobulin A
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • Receptors, Fc
  • Receptors, IgE
  • Receptors, IgG
  • Receptors, Transferrin
  • Interleukin-10
  • Interleukin-12