Overexpression of Ang-2 mRNA in non-small cell lung cancer: association with angiogenesis and poor prognosis

Oncol Rep. 2004 Oct;12(4):849-53.


Overexpressed Angiopoietin-2 (Ang-2) derived mainly from cancer cells was reported to promote tumor angiogenesis. The aim of this study was to evaluate the expression of Ang-2 in non-small cell lung cancers (NSCLCs). We investigated Ang-2 expression in 77 patients with NSCLC who underwent curative tumor resection by means of reverse transcription-polymerase chain reaction (RT-PCR). We also determined whether or not expression of Ang-2 mRNA correlates with immunohistochemical assays of Ang-2 protein and microvessel density (MVD) level. The level of Ang-2 mRNA expression was presented by the relative yield of each gene to the S14 mRNA, respectively. Ang-2 mRNA expression in NSCLC was significantly greater than that in non-cancerous normal lung (p=0.0178). The Ang-2 mRNA expression was significantly associated with lymph node metastasis, stage, Ang-2 protein, and microvessel density (MVD) level (p=0.0009 for lymph node metastasis; p=0.0002 for stage; p<0.0001 for Ang-2 protein; p<0.0001 for MVD). With regard to prognosis, the overall and stage I survival rates for patients in the high Ang-2 mRNA expression group were significantly poorer when compared with the low Ang-2 mRNA expression group (p<0.0001 for overall; p=0.0201 for stage I). Furthermore, expression of Ang-2 mRNA was an independent predictor of prognosis by multivariate analysis (p=0.0028). These data indicate that Ang-2 may contribute to tumor angiogenesis and progression and that Ang-2 gene expression can serve as a useful prognostic marker in NSCLC.

Publication types

  • Comparative Study

MeSH terms

  • Adenocarcinoma / blood supply
  • Adenocarcinoma / genetics
  • Adenocarcinoma / metabolism
  • Adult
  • Aged
  • Aged, 80 and over
  • Angiopoietin-2 / genetics
  • Angiopoietin-2 / metabolism*
  • Carcinoma, Large Cell / blood supply
  • Carcinoma, Large Cell / genetics
  • Carcinoma, Large Cell / metabolism
  • Carcinoma, Non-Small-Cell Lung / blood supply
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Carcinoma, Non-Small-Cell Lung / metabolism
  • Carcinoma, Squamous Cell / blood supply
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / metabolism
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Lung Neoplasms / blood supply
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / metabolism
  • Lymphatic Metastasis
  • Male
  • Microcirculation
  • Middle Aged
  • Neoplasm Invasiveness
  • Neoplasm Staging
  • Neovascularization, Pathologic / genetics
  • Neovascularization, Pathologic / metabolism*
  • Nuclear Proteins
  • Prognosis
  • Proteins / genetics
  • Proteins / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Neoplasm
  • Reverse Transcriptase Polymerase Chain Reaction
  • Survival Rate
  • Transcription Factors


  • Angiopoietin-2
  • Nuclear Proteins
  • Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • THRSP protein, human
  • Transcription Factors