Effects of genetic and nutritional factors on bone mineral density in young adults

Int J Mol Med. 2004 Oct;14(4):669-76.


To estimate the genetic and dietary factors influencing bone mineral density (BMD) in young adults, a total of 53 healthy volunteers (HV) (age 20.89+/-1.34), from whom informed consent was obtained, answered a questionnaire on dietary factors and had DNA from peripheral blood mononuclear cells analyzed for single nucleotide polymorphisms (SNPs) for vitamin (Vit) D receptor (VDR), estrogen receptor alpha (ERalpha), interleukin 1 receptor antagonist (IL1RA), and apolipoprotein E (ApoE) genes. Daily intakes of Vit C, fiber, soybean and related foods, and green and yellow vegetables showed a correlation with % BMD. In addition, Vit B2 as well as Vit C, and vegetables were identified as important factors for BMD by Stepwise regression analysis. Among the SNPs analyzed, the B+ type of the VDR gene tended to be associated with a lower BMD, and pp type of the ER gene digested by the PvuII enzyme in females indicated a significantly lower BMD than that in males. In addition, these SNPs were also identified by factor analysis to be associated with BMD. These results suggested that a complex array of genetic factors, such as two or more SNPs or SNPs and gender, may be important to BMD.

MeSH terms

  • Adult
  • Apolipoproteins E / genetics
  • Bone Density / genetics*
  • Bone Density / physiology*
  • Diet*
  • Estrogen Receptor alpha / genetics
  • Female
  • Humans
  • Interleukin 1 Receptor Antagonist Protein
  • Linear Models
  • Male
  • Nutritional Physiological Phenomena
  • Nutritional Status / physiology*
  • Polymorphism, Single Nucleotide / genetics
  • Receptors, Calcitriol / genetics
  • Sex Characteristics
  • Sialoglycoproteins / genetics
  • Surveys and Questionnaires


  • Apolipoproteins E
  • Estrogen Receptor alpha
  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Receptors, Calcitriol
  • Sialoglycoproteins