Identification of a human cDNA with high homology to yeast omnipotent suppressor 45

Gene. 1992 Jan 15;110(2):239-43. doi: 10.1016/0378-1119(92)90655-9.


Omnipotent suppression is a well-established phenomenon in yeast and bacteria in which nonsense mutations are misread. Wild-type (wt) suppressors are presumed to be involved in ensuring the fidelity of translation. We report a human homolog to wt yeast omnipotent suppressor 45 which shares 63% identity at the nucleotide level in the area of open reading frame (ORF) and 73% similarity at the amino acid (aa) level. The aa sequence of the human protein was deduced from a 2.3-kb cDNA (TB3-1) isolated from an adenocarcinoma T84 cell line cDNA library. The cDNA contains an ORF of 1284 bp which encodes a 47.8-kDa protein. Two transcripts for the clone were identified (2.6 and 4.0 kb) in a variety of human cell types. The strong structural similarity to yeast omnipotent suppressor 45, and its widespread expression suggest that this cDNA may play a role in the accurate recognition of nonsense codons in mammalian cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Codon / genetics
  • DNA, Recombinant / genetics
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Humans
  • Molecular Sequence Data
  • Protein Biosynthesis / genetics*
  • Proteins / chemistry
  • Proteins / genetics*
  • Ribosomal Proteins / genetics
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Suppression, Genetic*
  • Tumor Cells, Cultured


  • Codon
  • DNA, Recombinant
  • Fungal Proteins
  • Proteins
  • Ribosomal Proteins

Associated data

  • GENBANK/M64424
  • GENBANK/M64425
  • GENBANK/M64426
  • GENBANK/M64427
  • GENBANK/M75715
  • GENBANK/S39996
  • GENBANK/S40001
  • GENBANK/S40008
  • GENBANK/S40009
  • GENBANK/S40010