Computed tomography monitoring of radiation-induced lung fibrosis in mice

Invest Radiol. 2004 Oct;39(10):600-9. doi: 10.1097/01.rli.0000138134.89050.a5.


Rationale and objectives: To identify characteristics of lung fibrosis in a mouse model after radiotherapy (RT) using thin-section computed tomography (CT), histology and clinical parameters.

Materials and method: Using a multislice CT-scanner, follow-up chest CT scans of 10 out of 72 included mice (C57BL/6J, 36 control mice, 36 mice (20Gy)) were performed every 2 weeks until week 26 after RT. Hounsfield units (HU) and cardiothoracic ratio (CTR) were measured, and a multireader analysis on characteristic lung changes was performed and correlated with histology and clinical parameters.

Results: From weeks 4 to 8 after RT changes in histology (leukocyte count, extraalveolar edema, P < 0.01) and from week 12 changes in CT were detected (increase in HU, intralobular opacity and fibrotic strandings, P < 0.05). From week 14 clinical manifestations occurred (loss of weight, mobility, breathing, increased mortality, P < 0.01). CTR showed no significant changes. Three readers showed excellent interobserver agreement (kappa >0.84).

Conclusion: Thin-section CT in a mouse model is capable of detecting the development of lung fibrosis after RT prior to the onset of clinical deterioration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Edema
  • Female
  • Histological Techniques
  • Leukocyte Count
  • Mice
  • Mice, Inbred C57BL
  • Models, Animal
  • Monitoring, Physiologic
  • Pulmonary Fibrosis / diagnostic imaging*
  • Pulmonary Fibrosis / physiopathology*
  • Radiation Injuries, Experimental / physiopathology*
  • Thorax / radiation effects
  • Tomography, X-Ray Computed*