Induction of Mycobacterium avium proteins upon infection of human macrophages

Proteomics. 2004 Oct;4(10):3078-83. doi: 10.1002/pmic.200300891.


Induction of Mycobacterium avium proteins labelled with [35S]methionine and mRNAs upon infection of the human macrophage cell line THP-1 was investigated by two-dimensional gel electrophoresis-mass spectrometry and reverse transcriptase-polymerase chain reaction (RT-PCR), respectively. M. avium overexpressed proteins within the macrophages that are involved in fatty acids metabolism (FadE2, FixA), cell wall synthesis (KasA), and protein synthesis (EF-tu). The correlation of differential protein and mRNA expression varied between good and no correlation. Overall, these four proteins may be involved in the adaptation and survival of M. avium within human macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Cell Line
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Macrophages / microbiology*
  • Mycobacterium Infections / metabolism
  • Mycobacterium avium / metabolism*
  • Oligonucleotides / chemistry
  • Peptide Elongation Factor Tu / chemistry
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization


  • Bacterial Proteins
  • Oligonucleotides
  • RNA, Messenger
  • Peptide Elongation Factor Tu