The complete nucleotide sequence and its organization of the genome of Barley yellow dwarf virus-GAV

Sci China C Life Sci. 2004 Apr;47(2):175-82. doi: 10.1360/03yc0076.


The complete nucleotide sequence of genomic RNA of BYDV-GAV was determined. It comprised 5685 nucleotides and contained six open reading frames and four un-translated regions. The size and organization of BYDV-GAV genome were similar to those of BYDV PAV-aus. The nucleotide and deduced amino acid sequences of the six ORFs were aligned and compared with those of other luteoviruses. The results showed that there was a high degree of identity between BYDV-GAV and MAV-PS1 in all ORFs except ORF5 and ORF6, which had only 87.4% and 70.2% identities respectively. The reported genomic nucleotide sequence of MAV was shorter than that of BYDV-GAV, but the comparison of the genomic nucleotide sequences for MAV-PS1 and GAV showed 90.4% sequence identity for the same region of the genome. According to the level of sequence similarities, BYDV-GAV should be closely related to BYDV-MAV.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Viral / chemistry
  • DNA, Viral / genetics
  • Hordeum / virology*
  • Luteovirus / classification
  • Luteovirus / genetics*
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment


  • DNA, Complementary
  • DNA, Viral