Functional recovery of stroke rats induced by granulocyte colony-stimulating factor-stimulated stem cells

Circulation. 2004 Sep 28;110(13):1847-54. doi: 10.1161/01.CIR.0000142616.07367.66. Epub 2004 Sep 20.


Background: Stroke is a leading cause of death and disability worldwide; however, no effective treatment currently exists.

Methods and results: Rats receiving subcutaneous granulocyte colony-stimulating factor (G-CSF) showed less cerebral infarction, as evaluated by MRI, and improved motor performance after right middle cerebral artery ligation than vehicle-treated control rats. Subcutaneous administration of G-CSF enhanced the availability of circulating hematopoietic stem cells to the brain and their capacity for neurogenesis and angiogenesis in rats with cerebral ischemia.

Conclusions: G-CSF induced increases in bone marrow cell mobilization and targeting to the brain, reducing the volume of cerebral infarction and improving neural plasticity and vascularization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers
  • Bone Marrow / drug effects
  • Brain Chemistry
  • Cell Differentiation
  • Cell Division
  • Cell Lineage
  • DNA Replication
  • Drug Evaluation, Preclinical
  • Granulocyte Colony-Stimulating Factor / administration & dosage
  • Granulocyte Colony-Stimulating Factor / therapeutic use*
  • Head Movements
  • Hematopoietic Stem Cell Mobilization*
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / drug effects*
  • Infarction, Middle Cerebral Artery / physiopathology
  • Infarction, Middle Cerebral Artery / therapy*
  • Injections, Subcutaneous
  • Locomotion
  • Male
  • Neovascularization, Physiologic / drug effects
  • Neuronal Plasticity / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, CXCR4 / biosynthesis
  • Receptors, CXCR4 / genetics
  • Recovery of Function
  • Up-Regulation


  • Biomarkers
  • Cxcr4 protein, rat
  • Receptors, CXCR4
  • Granulocyte Colony-Stimulating Factor