Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis
- PMID: 15381864
- PMCID: PMC2717366
- DOI: 10.3347/kjp.2004.42.3.145
Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis
Abstract
We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.
Comment in
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Double strand problems: reverse DNA sequences deposited in the DNA database.Korean J Parasitol. 2010 Mar;48(1):89-90; author reply 91. doi: 10.3347/kjp.2010.48.1.89. Epub 2010 Mar 18. Korean J Parasitol. 2010. PMID: 20333294 Free PMC article. No abstract available.
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References
-
- Ando K, Sithithaworn P, Nuchjunggreed C, et al. Nucleotide sequence of mitochondrial COI and ribosomal ITSII of Opisthorchis viverrini in Northeast Thailand. Southeast Asian J Trop Med Public Health. 2001;32:17–22. - PubMed
-
- Barker SC, Blair D. Molecular phylogeny of Schistosoma species supports traditional groupings within the genus. J Parasitol. 1996;82:292–298. - PubMed
-
- Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992;54:165–173. - PubMed
-
- Bowles J, Blair D, McManus DP. A molecular phylogeny of the human schistosomes. Mol Phylogenet Evol. 1995;4:103–109. - PubMed
-
- Bowles J, Hope M, Tiu WU, Liu X, McManus DP. Nuclear and mitochondrial genetic markers highly conserved between Chinese and Philippine Schistosoma japonicum. Acta Trop. 1993;55:217–229. - PubMed
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