Prognostic factors in bladder carcinoma: histologic parameters and expression of a cell cycle-related nuclear antigen (Ki-67)

J Pathol. 1992 Jan;166(1):37-43. doi: 10.1002/path.1711660107.


A total of 316 biopsies from patients with bladder carcinoma who entered a trial of the genito-urinary group of the European Organization for Research and Treatment of Cancer (EORTC) were reviewed. The histological data were subsequently correlated with the clinical course. A strong correlation between the number of mitoses and the time of first recurrence, muscle invasion, and death was noted. Next the expression of Ki-67 in frozen sections from 49 transitional cell carcinomas (TCCs) and the DNA content of the tumour cells were determined. The frequency of Ki-67-positive tumour cells increased with tumour grade and stage. Grade II TCCs and superficially infiltrating TCCs showed a wide range of Ki-67 scores. There was a significant difference in Ki-67 score between non-infiltrating (Ta) and superficially infiltrating (T1) grade II TCCs. All DNA-aneuploid carcinomas but also 15 out of 36 DNA-diploid tumours contained more than 10 per cent Ki-67-positive cells. Only some of these tumours were DNA-aneuploid or -tetraploid. The results indicate that the number of Ki-67-positive cells in grade II tumours may be a useful aid in separating grade II TCCs with a favourable prognosis from those with a poor clinical outcome.

MeSH terms

  • Antigens, Neoplasm / analysis*
  • Antigens, Nuclear
  • Carcinoma, Transitional Cell / immunology
  • Carcinoma, Transitional Cell / pathology*
  • Cell Division / physiology
  • Humans
  • Immunoenzyme Techniques
  • Mitosis / physiology
  • Nuclear Proteins / analysis*
  • Prognosis
  • Urinary Bladder Neoplasms / immunology
  • Urinary Bladder Neoplasms / pathology*


  • Antigens, Neoplasm
  • Antigens, Nuclear
  • Nuclear Proteins