Characterization of Glucose Uptake by Cultured Rat Podocytes

Kidney Blood Press Res. 2005;28(1):1-7. doi: 10.1159/000080889. Epub 2004 Sep 20.

Abstract

The nonmetabolizable glucose analogue [(3)H]-2-deoxy-D-glucose ((3)H-2DG) was used to study glucose transport in cultured rat podocytes. Intracellular accumulation of (3)H-2DG was linear up to 20 min and was inhibited by cytochalasin B (80% inhibition) and by phlorizin (20% inhibition). Pretreatment with insulin stimulated the (3)H-2DG uptake 1.5-fold. A Hill analysis of the rate of glucose transport yielded a V(max) value of approximately 10 mM and S(0.5)of 7.8 mM. The value h = 1.0 for a Hill coefficient confirmed that glucose uptake exhibited a Michaelis-Menten kinetics. Transporters GLUT2 and GLUT4 were expressed in over 90% podocytes. Of the GLUT2- and GLUT4-expressing cells, approximately one-fourth expressed the membrane-bound fraction. We conclude that cultured rat podocytes possess a differentiated glucose transport system consisting chiefly of facilitative GLUT2 and GLUT4 transporters. It seems likely that a sodium-dependent glucose cotransporter may also be present in these cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antimetabolites / pharmacology
  • Biological Transport / drug effects
  • Biological Transport / physiology
  • Cells, Cultured
  • Deoxyglucose / pharmacology
  • Female
  • Glucose / metabolism*
  • Glucose Transporter Type 2 / metabolism
  • Glucose Transporter Type 4 / metabolism
  • Hypoglycemic Agents / pharmacology
  • Insulin / pharmacology
  • Podocytes / cytology
  • Podocytes / metabolism*
  • Rats
  • Rats, Wistar

Substances

  • Antimetabolites
  • Glucose Transporter Type 2
  • Glucose Transporter Type 4
  • Hypoglycemic Agents
  • Insulin
  • Deoxyglucose
  • Glucose