RNA-based gene transfer for adult stem cells and T cells

Leukemia. 2004 Nov;18(11):1898-902. doi: 10.1038/sj.leu.2403463.


Electroporation of mRNA has become an established method for gene transfer into dendritic cells for immunotherapeutic purposes. However, many more cell types and applications might benefit from an efficient mRNA-based gene transfer method. In this study, we investigated the potential of mRNA-based gene transfer to induce short-term transgene expression in adult stem cells and activated T cells, based on electroporation with mRNA encoding the enhanced green fluorescent protein. The results show efficient transgene expression in CD34-positive hematopoietic progenitor cells (35%), in in vitro cultured mesenchymal cells (90%) and in PHA-stimulated T cells (50%). Next to presentation of gene transfer results, potential applications of mRNA-based gene transfer in stem cells and T cells are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, CD34 / metabolism
  • Electroporation / standards*
  • Gene Transfer Techniques / standards*
  • Hematopoietic Stem Cells / metabolism*
  • Herpesvirus 8, Human / genetics
  • Humans
  • Lymphocyte Activation
  • Mesoderm / metabolism
  • Phytohemagglutinins / pharmacology
  • RNA, Messenger / genetics*
  • T-Lymphocytes / metabolism*
  • Transgenes / physiology*


  • Antigens, CD34
  • Phytohemagglutinins
  • RNA, Messenger