Oxidative Stress and Serum Paraoxonase Activity in Experimental Hypothyroidism: Effect of Vitamin E Supplementation

Cell Biochem Funct. Jan-Feb 2005;23(1):1-8. doi: 10.1002/cbf.1119.


Thyroid hormones are associated with the oxidative and antioxidative status of the organism. Since data on the oxidative status of hypothyroidism are limited and controversial, we investigated the oxidant and antioxidant status and serum paraoxonase/arylesterase activities in propylthiouracil-induced hypothyroidism and examined the effect of vitamin E supplementation on this experimental model. Forty male Sprague Dawley rats were randomly divided into four groups (group 1, control; group 2, control + vitamin E; group 3, propylthiouracil; group 4, propylthiouracil + vitamin E). Plasma, red blood cell, liver, heart and skeletal muscle malondialdehyde levels were increased in the propylthiouracil-treated group compared with the control rats and were decreased in propylthiouracil + vitamin E group compared with the propylthiouracil-treated group. Vitamin E supplementation also significantly increased liver and kidney reduced glutathione levels in propylthiouracil treated animals. Serum paraoxonase and arylesterase activities were decreased in propylthiouracil treated group and vitamin E supplementation caused significant increase in serum paraoxonase activity compared with the propylthiouracil-treated rats. These findings suggest that hypothyroidism is accompanied with increased oxidative stress and vitamin E supplementation exerts beneficial effects on this situation.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / metabolism
  • Antioxidants / pharmacology
  • Aryldialkylphosphatase / blood
  • Aryldialkylphosphatase / drug effects
  • Aryldialkylphosphatase / metabolism*
  • Body Weight
  • Dietary Supplements*
  • Disease Models, Animal
  • Hypothyroidism / blood
  • Hypothyroidism / chemically induced
  • Hypothyroidism / metabolism*
  • In Vitro Techniques
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects*
  • Oxidative Stress / physiology
  • Propylthiouracil
  • Rats
  • Rats, Sprague-Dawley
  • Thyroid Hormones / metabolism
  • Vitamin E / administration & dosage
  • Vitamin E / blood
  • Vitamin E / pharmacology*


  • Antioxidants
  • Thyroid Hormones
  • Vitamin E
  • Malondialdehyde
  • Propylthiouracil
  • Aryldialkylphosphatase