Inhibitory effects of mulberry leaf extract on postprandial hyperglycemia in normal rats

J Nutr Sci Vitaminol (Tokyo). 2004 Jun;50(3):161-4. doi: 10.3177/jnsv.50.161.


We examined the inhibitory effects of aqueous ethanol extract from mulberry leaves (ME) on postprandial hyperglycemia in normal Wistar rats. ME dose-dependently suppressed the postprandial rise of blood glucose in rats, when ME (0.02-0.5 g/kg) was given 0.5 h before the administration of carbohydrates such as sucrose, maltose and starch. The ME dose showing 50% inhibition of the increment of blood glucose (ED50) was 0.11 g/kg for sucrose, 0.44 g/kg for maltose, and 0.38 g/kg for starch. ME and its basic fraction (MB) containing 1-deoxynojirimycin were assayed for their inhibitory effects (IC50) on disaccharidase derived from the small intestine of rats. The IC50 value of ME was 3.2 microg/mL for sucrase, 10 microg/mL for isomaltase, and 51 microg/mL for maltase. The IC50 value of MB was 0.36 microg/mL for sucrase, 1.1 microg/mL for isomaltase, and 6.2 microg/mL for maltase. The IC50 value of 1-deoxynojirimycin as the principle component in ME was 0.015 microg/mL for sucrase and 0.21 microg/mL for maltase, and this value was comparable to the IC50 of voglibose. The inhibitory activity of ME in a-amylase was weak. These results suggest that ME strongly suppresses postprandial hyperglycemia after carbohydrate loading by inhibiting the activity of disaccharidases in the small intestine of rats.

MeSH terms

  • 1-Deoxynojirimycin / pharmacology
  • Animals
  • Dietary Carbohydrates / administration & dosage
  • Dietary Sucrose / administration & dosage
  • Disaccharidases / antagonists & inhibitors
  • Enzyme Inhibitors / pharmacology
  • Food*
  • Glycoside Hydrolase Inhibitors
  • Hyperglycemia / prevention & control*
  • Intestine, Small / enzymology
  • Maltose / administration & dosage
  • Morus / chemistry*
  • Oligo-1,6-Glucosidase / antagonists & inhibitors
  • Plant Extracts / administration & dosage*
  • Plant Leaves / chemistry*
  • Rats
  • Rats, Wistar
  • Starch / administration & dosage
  • Sucrase / antagonists & inhibitors


  • Dietary Carbohydrates
  • Dietary Sucrose
  • Enzyme Inhibitors
  • Glycoside Hydrolase Inhibitors
  • Plant Extracts
  • 1-Deoxynojirimycin
  • Maltose
  • Starch
  • Disaccharidases
  • Oligo-1,6-Glucosidase
  • Sucrase