Cloning and domain structure of the mammalian splicing factor U2AF

P D Zamore; J G Patton; M R Green

doi:10.1038/355609a0

Cloning and domain structure of the mammalian splicing factor U2AF

Nature. 1992 Feb 13;355(6361):609-14. doi: 10.1038/355609a0.

Authors

P D Zamore¹, J G Patton, M R Green

Affiliation

¹ Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

PMID: 1538748
DOI: 10.1038/355609a0

Abstract

A complementary DNA clone encoding the large subunit of the essential mammalian pre-messenger RNA splicing component U2 snRNP auxiliary factor (U2AF65) has been isolated and expressed in vitro. It contains two functional domains: a sequence-specific RNA-binding region composed of three ribonucleoprotein-consensus sequence domains, and an arginine/serine-rich motif necessary for splicing but not for binding to pre-mRNA.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Chromosome Mapping
Cloning, Molecular
DNA / isolation & purification
Endoribonucleases / genetics*
Genetic Complementation Test
Humans
Mammals
Molecular Sequence Data
Open Reading Frames
RNA Splicing
RNA, Messenger / analysis
RNA, Messenger / metabolism

Substances

RNA, Messenger
DNA
Endoribonucleases
ribonuclease U2