Abstract
To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Cell Line
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Enzyme Precursors / genetics
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Enzyme Precursors / metabolism
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Humans
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Intracellular Signaling Peptides and Proteins
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MAP Kinase Signaling System / physiology
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Oncogene Proteins / metabolism
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Phosphatidylinositol 3-Kinases / genetics
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Phosphatidylinositol 3-Kinases / metabolism*
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Phosphorylation
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Protein Binding
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Protein Structure, Tertiary
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Protein-Tyrosine Kinases / genetics
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Protein-Tyrosine Kinases / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Syk Kinase
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Two-Hybrid System Techniques
Substances
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Adaptor Proteins, Signal Transducing
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Enzyme Precursors
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Intracellular Signaling Peptides and Proteins
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Nck protein
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Oncogene Proteins
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Phosphoproteins
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Recombinant Fusion Proteins
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SLP-76 signal Transducing adaptor proteins
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Phosphatidylinositol 3-Kinases
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Protein-Tyrosine Kinases
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SYK protein, human
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Syk Kinase
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emt protein-tyrosine kinase