Kangiella koreensis gen. nov., sp. nov. and Kangiella aquimarina sp. nov., isolated from a tidal flat of the Yellow Sea in Korea

Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1829-1835. doi: 10.1099/ijs.0.63156-0.

Abstract

Two Gram-negative, non-motile, non-spore-forming, rod-shaped organisms, strains SW-125T and SW-154T, were isolated from tidal flat sediment of the Yellow Sea in Korea, and subjected to a polyphasic taxonomic study. Strains SW-125T and SW-154T grew optimally at 30-37 degrees C and in the presence of 2-3 % (w/v) NaCl. They contained ubiquinone-8 (Q-8) as the predominant respiratory lipoquinone and iso-C(15 : 0) as the major fatty acid. The DNA G + C contents of strains SW-125T and SW-154T were 44 mol%. Phylogenetic trees based on 16S rRNA gene sequences revealed that the two strains form deep evolutionary lineages of descent within the gamma-Proteobacteria. Strains SW-125T and SW-154T exhibited 16S rRNA gene sequence similarity levels of less than 90 % to members of the gamma-Proteobacteria used in this analysis. Strains SW-125T and SW-154T showed a 16S rRNA gene sequence similarity level of 98.5 % and a mean DNA-DNA relatedness level of 9.4 %. Therefore, on the basis of phenotypic, phylogenetic and genomic data, a new genus, Kangiella gen. nov., is proposed to accommodate the novel strains, comprising two novel species, Kangiella koreensis sp. nov. (type strain, SW-125T = KCTC 12182T = DSM 16069T) and Kangiella aquimarina sp. nov. (type strain, SW-154T = KCTC 12183T = DSM 16071T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / isolation & purification
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / isolation & purification
  • Fatty Acids / analysis
  • Gammaproteobacteria / classification*
  • Gammaproteobacteria / genetics
  • Gammaproteobacteria / isolation & purification*
  • Gammaproteobacteria / physiology
  • Genes, rRNA
  • Growth Inhibitors / pharmacology
  • Korea
  • Molecular Sequence Data
  • Movement
  • Phylogeny
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Saline Solution, Hypertonic / pharmacology
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Spores, Bacterial
  • Temperature
  • Ubiquinone / analysis
  • Water Microbiology

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Growth Inhibitors
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Saline Solution, Hypertonic
  • Ubiquinone
  • ubiquinone 8

Associated data

  • GENBANK/AY520560
  • GENBANK/AY520561