Identification of a SUMO-binding motif that recognizes SUMO-modified proteins

Proc Natl Acad Sci U S A. 2004 Oct 5;101(40):14373-8. doi: 10.1073/pnas.0403498101. Epub 2004 Sep 23.


Posttranslational modification by the ubiquitin homologue, small ubiquitin-like modifier 1 (SUMO-1), has been established as an important regulatory mechanism. However, in most cases it is not clear how sumoylation regulates various cellular functions. Emerging evidence suggests that sumoylation may play a general role in regulating protein-protein interactions, as shown in RanBP2/Nup358 and RanGAP1 interaction. In this study, we have defined an amino acid sequence motif that binds SUMO. This motif, V/I-X-V/I-V/I, was identified by NMR spectroscopic characterization of interactions among SUMO-1 and peptides derived from proteins that are known to bind SUMO or sumoylated proteins. This motif binds all SUMO paralogues (SUMO-1-3). Using site-directed mutagenesis, we also show that this SUMO-binding motif in RanBP2/Nup358 is responsible for the interaction between RanBP2/Nup358 and sumoylated RanGAP1. The SUMO-binding motif exists in nearly all proteins known to be involved in SUMO-dependent processes, suggesting its general role in sumoylation-dependent cellular functions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Binding Sites
  • Consensus Sequence
  • GTPase-Activating Proteins / chemistry
  • GTPase-Activating Proteins / metabolism
  • Humans
  • In Vitro Techniques
  • Macromolecular Substances
  • Models, Molecular
  • Molecular Chaperones
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nuclear Magnetic Resonance, Biomolecular
  • Nuclear Pore Complex Proteins / chemistry
  • Nuclear Pore Complex Proteins / metabolism
  • Protein Processing, Post-Translational
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • SUMO-1 Protein / chemistry
  • SUMO-1 Protein / metabolism*
  • Small Ubiquitin-Related Modifier Proteins / chemistry
  • Small Ubiquitin-Related Modifier Proteins / metabolism
  • Static Electricity


  • GTPase-Activating Proteins
  • Macromolecular Substances
  • Molecular Chaperones
  • Nuclear Pore Complex Proteins
  • RANGAP1 protein, human
  • Recombinant Proteins
  • SUMO-1 Protein
  • SUMO2 protein, human
  • Small Ubiquitin-Related Modifier Proteins
  • ran-binding protein 2