Antinuclear autoantibodies in prostate cancer: immunity to LEDGF/p75, a survival protein highly expressed in prostate tumors and cleaved during apoptosis

Prostate. 2005 Jan 1;62(1):14-26. doi: 10.1002/pros.20112.


Background: Cancer patients produce autoantibodies to self-proteins called tumor-associated antigens (TAA). These autoantibodies represent potentially valuable tools for identifying novel biomarkers and therapeutic targets. This study was designed to identify TAA in prostate cancer (PCa).

Methods: Serum autoantibodies to the survival protein lens epithelium-derived growth factor p75 (LEDGF/p75) were detected by immunofluorescence microscopy, ELISA, and immunoblotting. Expression of LEDGF/p75 in prostate cells and tumors was evaluated by immunoblotting or immunohistochemistry. Apoptotic cleavage of LEDGF/p75 was detected by immunoblotting.

Results: Anti-LEDGF/p75 autoantibodies were detected by ELISA in 18.4% of PCa patients and 5.5% of matched controls (P < 0.001) but not in patients with benign prostatic hyperplasia (BPH). LEDGF/p75 expression was detected in 93% of prostate tumors but not in normal prostate. Strong expression of the protein was observed in 61% of prostate tumors. Moderate to high expression was also detected in BPH tissue. Cleavage of LEDGF/p75 was detected in apoptotic prostate cells.

Conclusions: The high expression of LEDGF/p75 in prostate tumors and BPH could be induced by inflammation and oxidative stress. LEDGF/p75 cleavage fragments generated during prostate tumor cell death might trigger autoantibodies under inflammatory conditions in certain patients.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / immunology
  • Adenocarcinoma / pathology
  • Aged
  • Aged, 80 and over
  • Antibodies, Antinuclear / immunology*
  • Antigens, Neoplasm / immunology*
  • Apoptosis / immunology
  • Autoantibodies / immunology*
  • Biomarkers, Tumor / immunology*
  • Cell Line, Tumor
  • Humans
  • Intercellular Signaling Peptides and Proteins / immunology*
  • Male
  • Middle Aged
  • Prostatic Neoplasms / immunology*
  • Prostatic Neoplasms / pathology
  • Rhabdomyosarcoma, Embryonal / immunology


  • Antibodies, Antinuclear
  • Antigens, Neoplasm
  • Autoantibodies
  • Biomarkers, Tumor
  • Intercellular Signaling Peptides and Proteins
  • lens epithelium-derived growth factor