Cloning of the Drosophila prospero gene and its expression in ganglion mother cells

Biochem Biophys Res Commun. 1992 Feb 14;182(3):1326-32. doi: 10.1016/0006-291x(92)91878-t.

Abstract

The Drosophila central nervous system comprises an enormous diversity of neurons that are originated from neuronal stem cells, neuroblasts. They generate a specific series of ganglion mother cells, each of which is once cleaved into a pair of neurons. Among genes known to control neurogenesis, prospero (pros) was recently identified as a gene required for gene expression specifying properties of some identified neurons. Here we report that pros encodes a nuclear protein containing a homeodomain-like sequence. In neuronal lineages of the central nervous system, pros protein is specifically detected in ganglion mother cells, although their parental neuroblasts have begun expressing a significant level of pros transcripts, suggesting a post-transcriptional control of pros expression. Our results provoke that in neuronal cell differentiation ganglion mother cells might play a pivotal role associating with the pros function.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Deletion
  • Cloning, Molecular
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / physiology
  • Ganglia / physiology
  • Gene Expression
  • Genes*
  • Immunohistochemistry
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Neurons / physiology
  • Nuclear Proteins / genetics*
  • Peptides / chemical synthesis
  • Peptides / immunology
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transcription Factors*
  • Transcription, Genetic

Substances

  • Drosophila Proteins
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Peptides
  • Transcription Factors
  • pros protein, Drosophila

Associated data

  • GENBANK/D10271
  • GENBANK/D10272
  • GENBANK/D10273
  • GENBANK/D10274
  • GENBANK/D10275
  • GENBANK/D10276
  • GENBANK/D10609
  • GENBANK/D90560
  • GENBANK/M84511
  • GENBANK/M84512
  • GENBANK/M84513