The inhibition of the oxidation of low density lipoprotein by (+)-catechin, a naturally occurring flavonoid

Biochem Pharmacol. 1992 Feb 4;43(3):445-50. doi: 10.1016/0006-2952(92)90562-w.

Abstract

(+)-Catechin inhibited the copper-catalysed oxidation of human low density lipoprotein (LDL) in a dose-dependent manner with complete inhibition at 20 micrograms/mL. The flavonoid at a concentration of 50 micrograms/mL also inhibited oxidation of LDL induced by the mouse transformed macrophage J774, human monocyte-derived macrophages and vascular endothelial cells isolated from human umbilical cords. LDL modified by copper-catalysed or cell-induced oxidation was endocytosed and degraded by human macrophages at a much greater rate than native LDL. LDL reisolated from copper or cell incubations in the presence of (+)-catechin was endocytosed and degraded at rates similar to native LDL. (+)-Catechin appeared to inhibit the uptake and degradation by macrophages of cell-modified LDL. The actions of (+)-catechin on cell-induced oxidation of LDL are consistent with the ability of flavonoids of similar structure to inhibit lipoxygenases and with a role for lipoxygenases in cell-induced modification of LDL in vivo.

MeSH terms

  • Animals
  • Catechin / pharmacology*
  • Cell Line, Transformed / drug effects
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism
  • Flavonoids / pharmacology*
  • Free Radical Scavengers
  • Humans
  • Lipoproteins / isolation & purification
  • Lipoproteins, LDL / metabolism*
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice
  • Monocytes / drug effects
  • Monocytes / metabolism
  • Oxidation-Reduction / drug effects

Substances

  • Flavonoids
  • Free Radical Scavengers
  • Lipoproteins
  • Lipoproteins, LDL
  • Catechin