Oligotyping of Italian celiac patients with the 11th International Histocompatibility Workshop reagents

Tissue Antigens. 1992 Jan;39(1):38-9. doi: 10.1111/j.1399-0039.1992.tb02155.x.

Abstract

Oligotyping for HLA-DRB1, DQA1 and DQB1 specificities has been performed on PCR-amplified DNA from 55 Italian celiac children, living in Rome, and 50 blood donors. 52.6% of CD patients were DR3;DQA1*0501;DQB1*0201-positive versus 14% of controls (RR = 6.85) and 34.5% were DR5,7;DQA1*DQB1*0201-positive versus 2% of controls (RR = 25.86). 7 patients (12.7%) were negative for the DQA1*0501/B1*0201 dimer: 3 of them were DR4 (5.4%) and the others typed as DR1,5; 1,7; 5,7 and w6,7. No patient was negative for both DQA1*0501 and DQB1*0201 alleles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Celiac Disease / genetics*
  • Child
  • Child, Preschool
  • DNA Probes, HLA / genetics
  • Genes, MHC Class II*
  • HLA-DQ Antigens / genetics*
  • HLA-DQ alpha-Chains
  • HLA-DQ beta-Chains
  • HLA-DR Antigens / genetics*
  • HLA-DRB1 Chains
  • Histocompatibility Antigens Class II / genetics*
  • Humans
  • Italy
  • Polymerase Chain Reaction

Substances

  • DNA Probes, HLA
  • HLA-DQ Antigens
  • HLA-DQ alpha-Chains
  • HLA-DQ beta-Chains
  • HLA-DQA1 antigen
  • HLA-DQB1 antigen
  • HLA-DR Antigens
  • HLA-DRB1 Chains
  • Histocompatibility Antigens Class II