A simple method of generating axenic derivatives of Dictyostelium strains

Exp Cell Res. 1992 Apr;199(2):383-6. doi: 10.1016/0014-4827(92)90449-i.


We describe a generally applicable method of adapting Dictyostelium from growth on a bacterial food source to axenic growth. Cells are initially selected by growth on a plastic substratum but subsequently acquire the ability to grow in suspension culture. These strains can be transformed efficiently by DNA-mediated gene transfer.

MeSH terms

  • Animals
  • Dictyostelium / growth & development*
  • Germ-Free Life*
  • Phenotype
  • Plasmids