Molecular cloning and nucleotide sequence determination of the regulator region of mecA gene in methicillin-resistant Staphylococcus aureus (MRSA)

FEBS Lett. 1992 Feb 24;298(2-3):133-6. doi: 10.1016/0014-5793(92)80039-j.


Molecular cloning and nucleotide sequence analysis were performed for the identification of the regulator genes of methicillin resistance in the genome of a MRSA strain N315. Two open reading frames (orfs) were identified in the 5'-flanking region of the mecA gene. Predicted amino acid sequences of these orfs showed extensive homology to the co-inducer and the repressor protein of the penicillinase (PCase) production in Staphylococcus aureus as well as in Bacillus licheniformis. These orfs are considered to encode putative co-inducer and repressor proteins specific for the regulation of methicillin resistance in MRSA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • DNA, Bacterial
  • Genes, Bacterial*
  • Genes, Regulator*
  • Hexosyltransferases*
  • Methicillin Resistance / genetics*
  • Molecular Sequence Data
  • Muramoylpentapeptide Carboxypeptidase / genetics*
  • Open Reading Frames
  • Penicillin-Binding Proteins
  • Peptidyl Transferases*
  • Restriction Mapping
  • Sequence Alignment
  • Staphylococcus aureus / genetics*


  • Bacterial Proteins
  • Carrier Proteins
  • DNA, Bacterial
  • Penicillin-Binding Proteins
  • Peptidyl Transferases
  • Hexosyltransferases
  • Muramoylpentapeptide Carboxypeptidase

Associated data

  • GENBANK/S90282
  • GENBANK/S90283
  • GENBANK/S90285
  • GENBANK/S90286
  • GENBANK/S90288
  • GENBANK/S90290
  • GENBANK/S90294
  • GENBANK/S90296
  • GENBANK/S90298
  • GENBANK/X63598