Reporter constructs with low background activity utilizing the cat gene

M Boshart; M Klüppel; A Schmidt; G Schütz; B Luckow

doi:10.1016/0378-1119(92)90456-y

Reporter constructs with low background activity utilizing the cat gene

Gene. 1992 Jan 2;110(1):129-30. doi: 10.1016/0378-1119(92)90456-y.

Authors

M Boshart¹, M Klüppel, A Schmidt, G Schütz, B Luckow

Affiliation

¹ Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg.

PMID: 1544570
DOI: 10.1016/0378-1119(92)90456-y

Abstract

Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. These plasmids minimize the background of transcription derived from cryptic promoters or cryptic regulatory elements within the vector.

MeSH terms

Animals
Base Sequence
Chloramphenicol O-Acetyltransferase / genetics*
Cloning, Molecular
Genes, Regulator
Genetic Vectors
Molecular Sequence Data
Plasmids
Promoter Regions, Genetic*
Rats
Tumor Cells, Cultured

Substances

Chloramphenicol O-Acetyltransferase

Associated data

GENBANK/M80483
GENBANK/M80484
GENBANK/S39996
GENBANK/S40001
GENBANK/S40008
GENBANK/S40009
GENBANK/S40010
GENBANK/S46736
GENBANK/S46737
GENBANK/X72580