Antinociceptive effect of different types of calcium channel inhibitors and the distribution of various calcium channel alpha 1 subunits in the dorsal horn of spinal cord in mice

Brain Res. 2004 Oct 22;1024(1-2):122-9. doi: 10.1016/j.brainres.2004.07.066.


To understand better which voltage-dependent calcium channels (VGCCs) are involved in nociceptive neurotransmission, we investigated the pharmacological properties and distribution of VGCCs in the mouse spinal cord. A behavioral assay revealed that intrathecal injections of omega-agatoxin TK, omega-agatoxin IVA, omega-conotoxin GVIA, and SNX-482, which block P/Q-, P/Q-, N-, and R-type calcium channels, respectively, produced analgesic effects, while an L-type channel blocker had no such effect. An electrophysiological study demonstrated the presence of various types of VGCCs within dorsal root ganglion (DRG) neurons. Immunohistochemistry revealed distinct localization of P/Q-, N-, L-, and R-type calcium channel subunits to the dorsal horn of the spinal cord. The results of this study revealed the localization and functions of several calcium channels that are involved in nociceptive neurotransmission within the dorsal horn of the mouse spinal cord.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analgesics / pharmacology*
  • Animals
  • Calcium Channel Blockers / pharmacology*
  • Calcium Channels / analysis
  • Calcium Channels / physiology*
  • In Vitro Techniques
  • Mice
  • Pain Measurement / drug effects
  • Pain Measurement / statistics & numerical data
  • Posterior Horn Cells / chemistry
  • Posterior Horn Cells / drug effects*
  • Posterior Horn Cells / physiology
  • Protein Subunits / analysis
  • Protein Subunits / antagonists & inhibitors*
  • Protein Subunits / physiology


  • Analgesics
  • Calcium Channel Blockers
  • Calcium Channels
  • Protein Subunits