The effects of sub-chronic exposure of Wistar rats to the herbicide Glyphosate-Biocarb

Toxicol Lett. 2004 Nov 2;153(2):227-32. doi: 10.1016/j.toxlet.2004.04.008.


The object of this study was to analyze the hepatic effects of the herbicide Glyphosate-Biocarb (as commercialized in Brazil) in Wistar rats. Animals were treated orally with water or 4.87, 48.7, or 487 mg/kg of glyphosate each 2 days, during 75 days. Sub-chronic treatment of animals starting from the lowest dose of glyphosate induced the leakage of hepatic intracellular enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), suggesting irreversible damage in hepatocytes. We observed the increase of Kupffer cells in hepatic sinusoid of glyphosate-treated animals. This was followed by large deposition of reticulin fibers, composed mainly of collagen type III. We may conclude that Glyphosate-Biocarb may induce hepatic histological changes as well as AST and ALT leaking from liver to serum in experimental models.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / blood
  • Animals
  • Aspartate Aminotransferases / blood
  • Biopsy
  • Collagen Type III / metabolism
  • Dose-Response Relationship, Drug
  • Glycine / analogs & derivatives*
  • Glycine / toxicity*
  • Glyphosate
  • Herbicides / toxicity*
  • Liver / drug effects*
  • Liver / pathology
  • Male
  • Rats
  • Rats, Wistar


  • Collagen Type III
  • Herbicides
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • Glycine