Phytate degradation by lactic acid bacteria and yeasts during the wholemeal dough fermentation: a 31P NMR study

J Agric Food Chem. 2004 Oct 6;52(20):6300-5. doi: 10.1021/jf049551p.


myo-Inositol hexaphosphate (IP6) is the main source of phosphorus in cereal grains, and therefore, in bakery products. Different microorganisms such as yeasts and lactic acid bacteria have phytase enzymes able to hydrolyze IP6 during the wholemeal breadmaking. In this paper, the phytase activity of Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus curvatus, and Saccharomyces cerevisiae strains, isolated from southern Italian sourdoughs, is assayed using the (31)P NMR technique. The sourdough technology based on the use of lactic acid bacteria in the breadmaking is finally suggested.

MeSH terms

  • 6-Phytase / metabolism*
  • Bread / microbiology
  • Food Handling
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Lactobacillus / enzymology*
  • Magnetic Resonance Spectroscopy
  • Phytic Acid / metabolism*
  • Random Amplified Polymorphic DNA Technique
  • Saccharomyces cerevisiae / enzymology*


  • Phytic Acid
  • 6-Phytase