Abstract
We demonstrate highly efficient evanescent-wave detection of fluorophore-labeled biomolecules in aqueous solutions positioned in the air holes of the microstructured part of a photonic crystal fiber. The air-suspended silica structures located between three neighboring air holes in the cladding crystal guide light with a large fraction of the optical field penetrating into the sample even at wavelengths in the visible range. An effective interaction length of several centimeters is obtained when a sample volume of less than 1 microL is used.
Publication types
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Evaluation Study
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Validation Study
MeSH terms
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Biocompatible Materials / chemistry*
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Biopolymers / analysis*
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Biopolymers / chemistry
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Colloids / analysis*
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Colloids / chemistry
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DNA / analysis*
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DNA / chemistry
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Microfluidics / instrumentation*
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Microfluidics / methods
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Porosity
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Solutions
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Spectrometry, Fluorescence / instrumentation*
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Spectrometry, Fluorescence / methods
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Ultrafiltration / instrumentation
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Ultrafiltration / methods
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Water / analysis
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Water / chemistry*
Substances
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Biocompatible Materials
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Biopolymers
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Colloids
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Solutions
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Water
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DNA