Canonical Wnt activity regulates trunk neural crest delamination linking BMP/noggin signaling with G1/S transition

Development. 2004 Nov;131(21):5327-39. doi: 10.1242/dev.01424. Epub 2004 Sep 29.

Abstract

Delamination of premigratory neural crest cells depends on a balance between BMP/noggin and on successful G1/S transition. Here, we report that BMP regulates G1/S transition and consequent crest delamination through canonical Wnt signaling. Noggin overexpression inhibits G1/S transition and blocking G1/S abrogates BMP-induced delamination; moreover, transcription of Wnt1 is stimulated by BMP and by the developing somites, which concomitantly inhibit noggin production. Interfering with beta-catenin and LEF/TCF inhibits G1/S transition, neural crest delamination and transcription of various BMP-dependent genes, which include Cad6B, Pax3 and Msx1, but not that of Slug, Sox9 or FoxD3. Hence, we propose that developing somites inhibit noggin transcription in the dorsal tube, resulting in activation of BMP and consequent Wnt1 production. Canonical Wnt signaling in turn stimulates G1/S transition and generation of neural crest cell motility independently of its proposed role in earlier neural crest specification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Morphogenetic Proteins / metabolism*
  • CHO Cells
  • Carrier Proteins
  • Chickens
  • Coculture Techniques
  • Cricetinae
  • Cyclin D1 / metabolism
  • Cytoskeletal Proteins / genetics
  • Cytoskeletal Proteins / metabolism
  • G1 Phase*
  • Gene Expression Regulation, Developmental
  • Lamins / metabolism
  • Neural Crest / cytology
  • Neural Crest / metabolism*
  • Proteins / genetics
  • Proteins / metabolism*
  • Proto-Oncogene Proteins / metabolism*
  • Quail
  • S Phase*
  • Signal Transduction*
  • Somites / metabolism
  • Trans-Activators / genetics
  • Trans-Activators / metabolism
  • Transcription, Genetic
  • Wnt Proteins
  • Wnt1 Protein
  • Xenopus
  • Xenopus Proteins
  • beta Catenin

Substances

  • Bone Morphogenetic Proteins
  • CTNNB1 protein, Xenopus
  • Carrier Proteins
  • Cytoskeletal Proteins
  • Lamins
  • Proteins
  • Proto-Oncogene Proteins
  • Trans-Activators
  • WNT1 protein, Xenopus
  • Wnt Proteins
  • Wnt1 Protein
  • Xenopus Proteins
  • beta Catenin
  • Cyclin D1
  • noggin protein