Daily ingestion of fermented milk containing Lactobacillus casei DN114001 improves innate-defense capacity in healthy middle-aged people

J Physiol Biochem. 2004 Jun;60(2):85-91. doi: 10.1007/BF03168444.


Different lactic acid bacteria have often been administered as a dietary means to enhance immune system activity. Based on this statement, the aim of the current work was to test the effects of a Lactobacillus casei DN114001 fermented milk consumption on the immune response capacity in middle-age volunteers. Forty-five healthy volunteers, 24 women and 21 men (aged: 51-58 years), were randomized into two groups to receive three cups per day of a L. casei DN114001 (10(8)-10(10) ufc/g) fermented milk (n = 23), or placebo (n = 22), during an 8-week period. Measurements were performed before (day 0), and after the nutritional intervention (day 56). After the trial, no changes in immune cell proportions were detected, but the probiotic-treated group increased oxidative burst capacity of monocytes (probiotic group: p = 0.029; placebo group: p = 0.625), as well as NK cells tumoricidal activity (probiotic group: p = 0.023; placebo group: p = 0.125). Results showed that daily intake of fermented milk containing Lactobacillus casei DN114001 could have a positive effect in modulating the innate immune defense in healthy-middle-age people.

Publication types

  • Clinical Trial
  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytotoxicity Tests, Immunologic
  • Diet*
  • Double-Blind Method
  • Female
  • Fermentation
  • Flow Cytometry
  • Humans
  • Immunity, Innate / drug effects*
  • K562 Cells
  • Killer Cells, Natural / drug effects
  • Killer Cells, Natural / immunology
  • Lactobacillus casei / metabolism*
  • Male
  • Middle Aged
  • Milk / microbiology*
  • Monocytes / drug effects
  • Monocytes / immunology
  • Placebos
  • Probiotics*
  • Respiratory Burst / drug effects
  • Time Factors


  • Placebos