Blimp-1 specifies neural crest and sensory neuron progenitors in the zebrafish embryo

Curr Biol. 2004 Oct 5;14(19):1772-7. doi: 10.1016/j.cub.2004.09.046.

Abstract

Developmental origins of the neural crest (NC), a quintessential and pluripotent vertebrate cell type, has historically been a topic of extensive investigation but continues to remain poorly understood. In the zebrafish embryo, NC and primary sensory neurons are thought to segregate from a common population of progenitor cells in response to lateral inhibition. Here, we show that the zebrafish homolog of the B-lymphocyte-induced maturation protein (Blimp-1) gene, u-boot (ubo), is induced by BMP signaling in cells at the boundary of the neural plate and nonneural ectoderm. Loss of Ubo activity not only inhibits specification of the NC but also impairs development of the primary sensory neurons. Conversely, misexpression of ubo results in the generation of supernumerary primary sensory neurons consistent with this cell type representing the default fate within the progenitor equivalence group. These results establish a link between the activity of the transcriptional regulator Blimp-1 and the inductive effects of BMP signaling in the inception of NC progenitor fate.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Morphogenetic Proteins / metabolism
  • Cell Differentiation / physiology*
  • DNA-Binding Proteins
  • Gene Expression Regulation, Developmental / physiology
  • Immunohistochemistry
  • In Situ Hybridization
  • Neural Crest / physiology*
  • Neurons, Afferent / physiology*
  • Nuclear Proteins
  • Positive Regulatory Domain I-Binding Factor 1
  • Repressor Proteins / metabolism
  • Repressor Proteins / physiology*
  • Signal Transduction / physiology
  • Transcription Factors / metabolism
  • Transcription Factors / physiology*
  • Zebrafish / embryology*
  • Zebrafish Proteins

Substances

  • Bone Morphogenetic Proteins
  • DNA-Binding Proteins
  • Nuclear Proteins
  • Repressor Proteins
  • Transcription Factors
  • Zebrafish Proteins
  • Positive Regulatory Domain I-Binding Factor 1
  • prdm1a protein, zebrafish