Isolation and characterization of alpha-glucosidase inhibitor from the fungus Ganoderma lucidum

J Microbiol. 2004 Sep;42(3):223-7.


An alpha-glucosidase inhibitor, SKG-3, was isolated from the fruiting bodies of Ganoderma lucidum and its physico-chemical properties were characterized. It was a highly specific and effective reversible inhibitor of alpha-glucosidase. It showed very potent inhibitory activity against alpha-glucosidase with an IC50 value of 4.6 micro g/ml, but no activity for any other glycosidases tested. Enzyme activity could be recovered upon dialysis, thus providing evidence for the reversibility of the inhibition. A Lineweaver-Burk plot indicated that the SKG-3 inhibition of alpha-glucosidase was competitive.

MeSH terms

  • Binding, Competitive
  • Drugs, Chinese Herbal / chemistry*
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / isolation & purification*
  • Enzyme Inhibitors / metabolism
  • Glycoside Hydrolase Inhibitors*
  • Inhibitory Concentration 50
  • Kinetics
  • Plant Extracts / chemistry*
  • Reishi / chemistry
  • Reishi / growth & development*
  • alpha-Glucosidases / metabolism


  • Drugs, Chinese Herbal
  • Enzyme Inhibitors
  • Glycoside Hydrolase Inhibitors
  • Plant Extracts
  • alpha-Glucosidases