Use of Simulated Blood Cultures for Antibiotic Effect on Time to Detection of the Two Blood Culture Systems BacT/ALERT and BACTEC 9240

New Microbiol. 2004 Jul;27(3):235-48.

Abstract

To avoid the influence of pre-analytical steps, this study was performed using sterile blood spiked with defined loads of microorganisms as inoculum. Time-to-Detection (TTD) was evaluated for the most frequently encountered bacteria comparing two commercially available blood culture systems, BD BACTEC 9240 (Becton Dickinson) and BacT/ALERT (Organon Teknika). The effect of the most widely used antibiotics on TTD was evaluated on both systems. TTD was measured with antibiotics at their trough and at increasing concentrations. The results show that the BACTEC PLUS system recovers more pathogens with shorter time to detection than the BacT/ALERT FAN system when beta-lactam antibiotics (Ampicillin, Cefotaxime) are present at their respective trough concentration corresponding to parenteral therapy. The two systems seem to be equally efficient when Gentamicin, Ciprofloxacin and Trimethoprim/sulfamethoxazole are used; in the case of Vancomycin, BACTEC seems more effective than BacT/ALERT.

Publication types

  • Evaluation Study

MeSH terms

  • Ampicillin / pharmacology
  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / pharmacology*
  • Bacteremia / diagnosis*
  • Bacteremia / microbiology
  • Bacteria / drug effects*
  • Bacteria / growth & development
  • Bacteria / isolation & purification*
  • Bacteriological Techniques / methods
  • Blood / microbiology*
  • Cefotaxime / pharmacology
  • Ciprofloxacin / pharmacology
  • Culture Media / chemistry
  • Enterococcus faecalis / drug effects
  • Enterococcus faecalis / growth & development
  • Enterococcus faecalis / isolation & purification
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development
  • Escherichia coli / isolation & purification
  • Gentamicins / pharmacology
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / growth & development
  • Staphylococcus aureus / isolation & purification
  • Streptococcus pneumoniae / drug effects
  • Streptococcus pneumoniae / growth & development
  • Streptococcus pneumoniae / isolation & purification
  • Time Factors
  • Trimethoprim, Sulfamethoxazole Drug Combination / pharmacology
  • Vancomycin / pharmacology

Substances

  • Anti-Bacterial Agents
  • Culture Media
  • Gentamicins
  • Ciprofloxacin
  • Vancomycin
  • Ampicillin
  • Trimethoprim, Sulfamethoxazole Drug Combination
  • Cefotaxime