Assessment of CFTR localisation in native airway epithelial cells obtained by nasal brushing

J Cyst Fibros. 2004 Aug:3 Suppl 2:43-8. doi: 10.1016/j.jcf.2004.05.009.

Abstract

Reliable methods for determining the localisation of mutant CFTR protein in native cells from CF individuals are necessary to allow the degree of mislocalisation of any genotype to be defined and to assess the effect of therapeutic agents on CFTR trafficking. Here, we present procedures for obtaining ciliated epithelial cells from CF patients by nasal brushing and a description of protocols for immunolocalisation of CFTR. The protocols are a consensus, following comparison of some aspects of methods currently used in the authors' laboratories.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cystic Fibrosis / diagnosis*
  • Cystic Fibrosis / genetics
  • Cystic Fibrosis Transmembrane Conductance Regulator / genetics
  • Cystic Fibrosis Transmembrane Conductance Regulator / isolation & purification*
  • Epithelial Cells / chemistry
  • Histocytological Preparation Techniques / methods*
  • Humans
  • Respiratory Mucosa / chemistry
  • Respiratory Mucosa / pathology*
  • Specimen Handling / methods

Substances

  • CFTR protein, human
  • Cystic Fibrosis Transmembrane Conductance Regulator