Bioremediation of textile azo dyes by aerobic bacterial consortium

Biodegradation. 2004 Aug;15(4):275-80. doi: 10.1023/b:biod.0000043000.18427.0a.


An aerobic bacterial consortium consisting of two isolated strains (BF1, BF2) and a strain of Pseudomonas putida (MTCC1194) was developed for the aerobic degradation of a mixture of textile azodyes and individual azodyes at alkaline pH (9-10.5) and salinity (0.9-3.68 g/l) at ambient temperature (28 +/- 2 degrees C). The degradation efficiency of the strains in different media (mineral media and in the Simulated textile effluent (STE)) and at different dye concentrations were studied. The presence of a H2O2 independent oxidase-laccase (26.5 IU/ml) was found in the culture filtrate of the organism BF2. The analysis of the degraded products by TLC and HPLC, after the microbial treatment of the dyes showed the absence of amines and the presence of low molecular weight oxidative degradation products. The enzymes present in the crude supernatant was found to be reusable for the dye degradation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds / metabolism*
  • Bacteria, Aerobic / isolation & purification*
  • Bacteria, Aerobic / metabolism
  • Biodegradation, Environmental
  • Biomass
  • Coloring Agents / analysis
  • Coloring Agents / metabolism*
  • Culture Media
  • Hydrogen-Ion Concentration
  • Laccase / metabolism
  • Pseudomonas putida / isolation & purification
  • Pseudomonas putida / metabolism
  • Soil Microbiology
  • Soil Pollutants / metabolism*
  • Textiles / microbiology
  • Time Factors


  • Azo Compounds
  • Coloring Agents
  • Culture Media
  • Soil Pollutants
  • Laccase