In Silico Identification and Structural Features of Six New Genes Similar to MATER Specifically Expressed in the Oocyte

Biochem Biophys Res Commun. 2004 Nov 12;324(2):547-53. doi: 10.1016/j.bbrc.2004.09.086.

Abstract

In the present work, we have used the in silico subtraction methodology to identify six new mouse genes similar to NALP5/MATER, whose ESTs were represented almost exclusively in egg libraries. Five genes were selected for RT-PCR and/or in situ hybridization. These experiments confirmed their oocyte restricted expression. Five of these genes are localized on mouse chromosome 7, as is NALP5/MATER; among them, three are localized in a 300 kb cluster.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens / genetics*
  • Antigens / metabolism*
  • DNA, Complementary / metabolism
  • Egg Proteins / genetics*
  • Egg Proteins / metabolism*
  • Expressed Sequence Tags
  • Gene Library
  • In Situ Hybridization
  • Mice
  • Models, Genetic
  • Molecular Sequence Data
  • Multigene Family
  • Oocytes / metabolism*
  • Protein Structure, Tertiary
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Antigens
  • DNA, Complementary
  • Egg Proteins
  • Nalp5 protein, mouse
  • RNA, Messenger