PLP2/A4 interacts with CCR1 and stimulates migration of CCR1-expressing HOS cells

Biochem Biophys Res Commun. 2004 Nov 12;324(2):768-72. doi: 10.1016/j.bbrc.2004.09.118.

Abstract

Multiple CC chemokines bind to CCR1, which plays important roles in immune and inflammatory responses. To search for proteins involved in the CCR1 signaling pathway, we screened a yeast two-hybrid library using the cytoplasmic tail of CCR1 as the bait. One of the positive clones contained an open reading frame of 456bp, of which the nucleotide sequence was identical to that of proteolipid protein 2 (PLP2), also known as protein A4. Mammalian two-hybrid and coimmunoprecipitation analyses demonstrated the association of PLP2/A4 with CCR1. Indirect immunofluorescence analysis revealed that PLP2/A4 was predominantly located in plasma membrane and colocalized with CCR1 in transfected human HEK293 cells. In addition, focal staining of CCR1 appeared on the periphery of the membrane upon short exposure to Leukotactin-1(Lkn-1)/CCL15, a CCR1 agonist, and was costained with PLP2/A4 on the focal regions. PLP2/A4 mRNAs were detected in various cells such as U-937, HL-60, HEK293, and HOS cells. Overexpression of PLP2/A4 stimulated a twofold increase in the agonist-induced migration of HOS/CCR1 cells, implicating a functional role for PLP2/A4 in the chemotactic processes via CCR1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Western
  • Cell Line
  • Cell Line, Tumor
  • Cell Movement
  • Chemotaxis
  • Chloramphenicol O-Acetyltransferase / metabolism
  • Cytoplasm / metabolism
  • Fluorescent Antibody Technique, Indirect
  • HL-60 Cells
  • Humans
  • Immunoprecipitation
  • MARVEL Domain-Containing Proteins
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Open Reading Frames
  • Plasmids / metabolism
  • Protein Binding
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases / metabolism*
  • Proteolipids
  • RNA / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Saccharomyces cerevisiae / metabolism
  • Sarcoma / metabolism
  • Signal Transduction
  • Transfection
  • Two-Hybrid System Techniques
  • U937 Cells

Substances

  • MARVEL Domain-Containing Proteins
  • Membrane Proteins
  • PLP2 protein, human
  • Proteolipids
  • RNA
  • Chloramphenicol O-Acetyltransferase
  • SNF1-related protein kinases
  • Protein-Serine-Threonine Kinases