Identification of the mouse H-ficolin gene as a pseudogene and orthology between mouse ficolins A/B and human L-/M-ficolins

Genomics. 2004 Oct;84(4):737-44. doi: 10.1016/j.ygeno.2004.07.006.

Abstract

Ficolin is a collagenous lectin which plays a crucial role in innate immunity. Three and two ficolins have been identified in human and mice, respectively. To identify the mouse homologue of human H-ficolin and to elucidate the orthology between mouse ficolins A/B and human L-/M-ficolins, the gene structures were explored. The mouse homologue of the H-ficolin gene was identified as a pseudogene on chromosome 4. The mouse ficolin A gene was located far from the ficolin B gene on chromosome 2, whereas the human L-ficolin and M-ficolin genes were close in the region homologous to the ficolin B locus. Together with the exon-intron structures and the phylogenetic tree, these results suggest that ficolin B is the mouse orthologue of M-ficolin and that the genes encoding serum-type ficolins, ficolin A and L-ficolin, were generated independently from the ficolin B/M-ficolin lineage each in mice and primates.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosomes / genetics
  • Cloning, Molecular
  • Exons / genetics*
  • Humans
  • Introns / genetics*
  • Lectins / classification
  • Lectins / genetics*
  • Lectins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred ICR
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • Pseudogenes / genetics*
  • Sequence Homology, Amino Acid

Substances

  • Lectins
  • ficolin

Associated data

  • GENBANK/AB175686
  • GENBANK/AB175687
  • GENBANK/AB175688