Ly-1 B (B-1) cells are the main source of B cell-derived interleukin 10

Eur J Immunol. 1992 Mar;22(3):711-7. doi: 10.1002/eji.1830220314.


We have previously reported (O'Garra, A. et al., Int. Immunol. 1990, 2:821) that murine B lymphomas and purified normal peritoneal B cells produce interleukin (IL) 10. We now show that this production of IL 10 B cells correlates with the presence of Ly-1 (B-1) B cells, in both normal and diseased mice. Using a semi-quantitative modification of the polymerase chain reaction, we show that IL 10 expression is detectable in peritoneal B cells but only becomes apparent in splenic B cells of aged mice of which a high proportion are Ly-1+. Furthermore, the expression of IL 10 is constitutive in splenic B cells from mice carrying the Ly-1+ BCL1 lymphoma. Since IL 10 is a potent regulator of in vitro immune function, its production by Ly-1 lineage B (B-1) cells raises the possibility that this subset of B cells may regulate their own development and/or the function of other immunocompetent cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / immunology
  • Animals
  • B-Lymphocytes / chemistry*
  • Base Sequence
  • Female
  • Interferon-gamma / analysis
  • Interleukin-10 / analysis*
  • Interleukin-10 / biosynthesis
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred CBA
  • Molecular Sequence Data
  • Spleen / chemistry


  • Interleukin-10
  • Interferon-gamma