Cytoprotective effect on oxidative stress and inhibitory effect on cellular aging of Terminalia chebula fruit

Phytother Res. 2004 Sep;18(9):737-41. doi: 10.1002/ptr.1529.


The ethanol extract from the fruit of Terminalia chebula (Combretaceae) exhibited significant inhibitory activity on oxidative stress and the age-dependent shortening of the telomeric DNA length. In the peroxidation model using t-BuOOH, the T. chebula extract showed a notable cytoprotective effect on the HEK-N/F cells with 60.5 +/- 3.8% at a concentration of 50 microg/ml. In addition, the T. chebula extract exhibited a significant cytoprotective effect against UVB-induced oxidative damage. The life-span of the HEK-N/F cells was elongated by 40% as a result of the continuous administration of 3 microg/ml of the T. chebula extract compared to that of the control. These observations were attributed to the inhibitory effect of the T. chebula extract on the age-dependent shortening of the telomere, length as shown by the Southern blots of the terminal restriction fragments (TRFs) of DNA extracted from subculture passages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / drug effects
  • Fruit
  • Humans
  • Oxidative Stress / drug effects
  • Phytotherapy*
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Protective Agents / administration & dosage
  • Protective Agents / pharmacology*
  • Protective Agents / therapeutic use
  • Skin Aging / drug effects*
  • Skin Aging / radiation effects
  • Telomere / drug effects
  • Terminalia*
  • Ultraviolet Rays


  • Plant Extracts
  • Protective Agents
  • DNA