Preservation of Rat Livers by Cold Storage: A Comparison Between the University of Wisconsin Solution and Hypothermosol

Ann Transplant. 2004;9(2):35-7.

Abstract

Objectives: The University of Wisconsin solution (UW) is the gold standard for cold storage (CS) of donor livers. However, UW contains the colloid Hydroxyethyl starch (HES), which may cause perfusion deficits due to its high viscosity. Recently, a new CS preservation solution, Hypothermosol (HTS), was introduced which contains the less viscous colloid Dextran. The aim of this study was to assess HTS as a cold storage solution for preservation of the liver.

Methods: In an isolated perfused rat liver model, hepatocellular damage was assessed after 24 hours of CS. Liver enzymes were measured during reperfusion with Krebs-Henseleit Buffer. Bile was collected during reperfusion as a parameter of liver function.

Results: CS using HTS showed a significant decrease of ALT and LDH levels (as compared to UW) at all time points during reperfusion. For LDH these results where most pronounced at t=10 min (84 +/- 7.09 vs 113 +/- 7.57: p < 0.05) and t=30 min (149.2 +/- 9.68 vs 194 +/-6.52: p< 0.05). Regarding liver function, more bile was produced after 24 hours CS in HTS, but this did not reach statistical significancy.

Conclusions: Cold storage preservation of rat livers using Hypothermosol results in equal or even better preservation as compared to cold storage using UW.

Publication types

  • Evaluation Study

MeSH terms

  • Adenosine / standards*
  • Alanine Transaminase / metabolism
  • Allopurinol / standards*
  • Animals
  • Bile / metabolism
  • Cryopreservation / standards*
  • Glutathione / standards*
  • Insulin / standards*
  • L-Lactate Dehydrogenase / metabolism
  • Liver* / enzymology*
  • Male
  • Organ Preservation Solutions / standards*
  • Raffinose / standards*
  • Rats
  • Rats, Wistar
  • Time Factors

Substances

  • Insulin
  • Organ Preservation Solutions
  • University of Wisconsin-lactobionate solution
  • hypothermosol
  • Allopurinol
  • L-Lactate Dehydrogenase
  • Alanine Transaminase
  • Glutathione
  • Adenosine
  • Raffinose