Novel antioxidant peptides from fermented mushroom Ganoderma lucidum

J Agric Food Chem. 2004 Oct 20;52(21):6646-52. doi: 10.1021/jf0495136.


Oxidative stress has been linked with the pathogenesis of many human diseases including cancer, aging, and atherosclerosis. The present study investigates the antioxidant activities of peptides isolated from the medicinal mushroom, Ganoderma lucidum. G. lucidum has been shown to possess potent antioxidant activity with little or no side effects. Polysaccharide, polysaccharide-peptide complex, and phenolic components of G. lucidum have been proposed to be responsible for this antioxidant effect. However, research has shown that the G. lucidum peptide (GLP) is the major antioxidant component of G. lucidum. The objective of this study was to evaluate the antioxidant activity of this peptide using different oxidation systems. GLP showed potent antioxidant activities in both lightproof soybean oil and lard systems, assessed by lipid peroxidant value. Compared to butylated hydroxytoluene, GLP showed a higher antioxidant activity in the soybean oil system. Soybean lipoxygenase activity was blocked by GLP in a dose-dependent manner with an IC50 value of 27.1 microg/mL. GLP showed scavenging activity toward hydroxyl radicals produced in a deoxyribose system with an IC50 value of 25 microg/mL, and GLP effectively quenched superoxide radical anion produced by pyrogallol autoxidation in a dose-dependent manner. Malondialdehyde level has been used as the oxidation index in many biological systems. GLP showed substantial antioxidant activity in the rat liver tissue homogenates and mitochondrial membrane peroxidation systems. The auto-hemolysis of rat red blood cells was also blocked by GLP in a dose-dependent manner. On the basis of these results, it is concluded that GLP is the major constituent responsible for the antioxidant activity of G. lucidum. GLP could play an important role in the inhibition of lipid peroxidation in biological systems through its antioxidant, metal chelating, and free radical scavenging activities.

MeSH terms

  • Animals
  • Antioxidants / analysis*
  • Antioxidants / pharmacology
  • Erythrocytes / metabolism
  • Fermentation
  • Hemolysis / drug effects
  • Lipid Peroxidation / drug effects
  • Lipoxygenase Inhibitors / pharmacology
  • Liver / chemistry
  • Male
  • Malondialdehyde / analysis
  • Mice
  • Mitochondria, Liver / chemistry
  • Mitochondria, Liver / metabolism
  • Oxidation-Reduction
  • Peptides / analysis*
  • Peptides / isolation & purification
  • Peptides / pharmacology
  • Rats
  • Rats, Wistar
  • Reishi / chemistry*
  • Soybeans / enzymology


  • Antioxidants
  • Lipoxygenase Inhibitors
  • Peptides
  • Malondialdehyde