Inter-simple sequence repeat markers and flow cytometry for the characterization of closely related Citrus limon germplasms

R Capparelli; M Viscardi; M G Amoroso; G Blaiotta; M Bianco

doi:10.1023/B:BILE.0000044920.36497.fc

Inter-simple sequence repeat markers and flow cytometry for the characterization of closely related Citrus limon germplasms

Biotechnol Lett. 2004 Aug;26(16):1295-9. doi: 10.1023/B:BILE.0000044920.36497.fc.

Authors

R Capparelli¹, M Viscardi, M G Amoroso, G Blaiotta, M Bianco

Affiliation

¹ Zootechnical Sciences and Food Inspection Department, School of Agriculture, University of Naples, Federico II, Via Università 133, Portici, 80055 Naples, Italy. capparel@unina.it

PMID: 15483390
DOI: 10.1023/B:BILE.0000044920.36497.fc

Abstract

The genetic relationship among commercial cultivars of Citrus limon (lemon) was analysed by inter-simple sequence repeats (ISSR) and flow cytometry techniques. Two cultivars with a close germplasm were distinguished by screening 10 SSR primers and by measuring DNA content of prestained nuclei.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Citrus / classification*
Citrus / genetics*
DNA, Plant / analysis*
DNA, Plant / genetics*
Flow Cytometry / methods*
Genetic Markers / genetics
Genome, Plant
Genotype
Repetitive Sequences, Nucleic Acid / genetics*
Self-Sustained Sequence Replication / methods*

Substances

DNA, Plant
Genetic Markers