HPLC quantification of metoprolol with solid-phase extraction for the drug monitoring of pediatric patients

Biomed Chromatogr. 2005 Apr;19(3):202-7. doi: 10.1002/bmc.436.


Although the analytical literature seems abundant for the determination of metoprolol in human plasma, a method using standard equipment providing a sensitive and simple high-performance liquid chromatographic (HPLC) method for limited blood volume, e.g. where 1 mL of blood in a 1 kg infant equals 70 mL of adult blood volume, has rarely been addressed. Therefore, in 500 microL of plasma, metoprolol was extracted using an internal standard and solid-phase extraction columns. Chromatographic analysis was performed on a Spherisorb C(6) column (5 microm particle size) at ambient temperature and fluorimetric detection with an excitation wavelength of 225 nm, and emission wavelength of 310 nm. The mobile phase [30% acetonitrile and 70% 0.25 m potassium acetate buffer (pH 4)] was pumped with 1 mL/min. Metoprolol recovery was determined at 73.0 +/- 20.5%, and the limit of quantitation was 2.4 ng/mL. Precision values of intra- and inter-assay were below 15.5% and those for accuracy were between 90 and 110%. This method was developed for monitoring and determination of pharmacokinetic parameters of metoprolol in pediatric patients and therefore metoprolol plasma concentrations in a 2-year-old child with ventricular tachycardia are reported. .

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Adult
  • Child
  • Child, Preschool
  • Chromatography, High Pressure Liquid / methods*
  • Drug Monitoring / methods*
  • Humans
  • Hydrogen-Ion Concentration
  • Metoprolol / blood*
  • Metoprolol / therapeutic use
  • Sensitivity and Specificity
  • Tachycardia, Ventricular / drug therapy


  • Metoprolol