Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody

J Med Virol. 2004 Dec;74(4):517-20. doi: 10.1002/jmv.20207.

Abstract

High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Child
  • Child, Preschool
  • Coronavirus / genetics
  • Coronavirus / isolation & purification*
  • Enzyme-Linked Immunosorbent Assay
  • Evaluation Studies as Topic
  • Female
  • Humans
  • Immunoglobulin G / analysis*
  • Immunoglobulin G / immunology
  • Infant
  • Male
  • Middle Aged
  • Nucleocapsid / analysis
  • SARS Virus / immunology*
  • Sensitivity and Specificity
  • Serologic Tests / methods
  • Severe Acute Respiratory Syndrome / diagnosis*
  • Severe Acute Respiratory Syndrome / epidemiology
  • Severe Acute Respiratory Syndrome / immunology*
  • Severe Acute Respiratory Syndrome / virology

Substances

  • Immunoglobulin G