Spectrophotometric Determination of Deacetylation Degree of Chitinous Materials Dissolved in Phosphoric Acid

Macromol Biosci. 2004 Oct 20;4(10):919-21. doi: 10.1002/mabi.200400084.


A simple spectrophotometric method is proposed for determining deacetylation degrees (DD) of chitinous materials using phosphoric acid as the UV-transparent solvent system. Calibrating by the extinction coefficients (A(210)) of D-glucosamine and N-acetyl-D-glucosamine, DD values (24-88%) were computed numerically. The results correlated well (R(2) = 0.9805, n = 50) with those obtained by solid-state (13)C NMR. Comparison of the results obtained by the proposed UV method and solid-state (13)C NMR.

MeSH terms

  • Acetylation
  • Chitin / chemistry*
  • Chitosan / chemistry
  • Magnetic Resonance Spectroscopy
  • Models, Theoretical
  • Phosphoric Acids / chemistry*
  • Software
  • Solvents
  • Spectrophotometry, Ultraviolet / methods*
  • Time Factors
  • Ultraviolet Rays


  • Phosphoric Acids
  • Solvents
  • Chitin
  • Chitosan
  • phosphoric acid