Evolutionary variation of the CCAAT-binding transcription factor NF-Y

Nucleic Acids Res. 1992 Mar 11;20(5):1087-91. doi: 10.1093/nar/20.5.1087.


NF-Y is a CCAAT-specific transcription factor thought to be involved in the regulation of a variety of eukaryotic genes. It shows a striking sequence similarity with the yeast factor HAP2/3. In an attempt to trace back its evolutionary history, we succeeded in isolating NF-Y cDNA clones from a plant and from several species of vertebrates. The patterns of sequence conservation delineate potential functional domains: A central, highly conserved, domain likely responsible for DNA-binding and subunit interaction; more evolutionarily flexible flanking regions, in which variability is clustered, individualizing conserved glutamine or acidic amino-acids putatively involved in protein-protein contacts.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution
  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • Fungi / genetics
  • Genetic Variation / genetics
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Structure-Activity Relationship
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Zea mays / genetics


  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • Transcription Factors

Associated data

  • GENBANK/X55315
  • GENBANK/X55316
  • GENBANK/X59709
  • GENBANK/X59710
  • GENBANK/X59711
  • GENBANK/X59712
  • GENBANK/X59713
  • GENBANK/X59714